Structure and genomic organization of the mouse dihydrofolate reductase gene

Jack H. Nunberg; Randal J. Kaufman; Annie C.Y. Chang; Stanley N. Cohen; Robert T. Schimke

doi:10.1016/0092-8674(80)90510-3

Structure and genomic organization of the mouse dihydrofolate reductase gene

Jack H. Nunberg, Randal J. Kaufman, Annie C.Y. Chang, Stanley N. Cohen, Robert T. Schimke

Montana Biotechnology Center

Stanford University

Research output: Contribution to journal › Article › peer-review

108 Scopus citations

Abstract

The genomic organization of the mouse dihydrofolate reductase gene has been determined by hybridization of specific cDNA sequences to restriction endonuclease-generated fragments of DNA from methotrexate-resistant S-180 cells. The dihydrofolate reductase gene contains a minimum of five intervening sequences (one in the 5′ untranslated region and four in the protein-coding region) and spans a minimum of 42 kilobase pairs on the genome. Genomic sequences at the junction of the intervening sequence and mRNA-coding sequence and at the polyadenylation site have been determined. A similar organization is found in independently isolated methotrexate-resistant cell lines, in the parental sensitive cell line and in several inbred mouse strains, indicating that this organization represents that of the natural gene.

Original language	English
Pages (from-to)	355-364
Number of pages	10
Journal	Cell
Volume	19
Issue number	2
DOIs	https://doi.org/10.1016/0092-8674(80)90510-3
State	Published - Feb 1980

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title = "Structure and genomic organization of the mouse dihydrofolate reductase gene",

abstract = "The genomic organization of the mouse dihydrofolate reductase gene has been determined by hybridization of specific cDNA sequences to restriction endonuclease-generated fragments of DNA from methotrexate-resistant S-180 cells. The dihydrofolate reductase gene contains a minimum of five intervening sequences (one in the 5′ untranslated region and four in the protein-coding region) and spans a minimum of 42 kilobase pairs on the genome. Genomic sequences at the junction of the intervening sequence and mRNA-coding sequence and at the polyadenylation site have been determined. A similar organization is found in independently isolated methotrexate-resistant cell lines, in the parental sensitive cell line and in several inbred mouse strains, indicating that this organization represents that of the natural gene.",

author = "Nunberg, {Jack H.} and Kaufman, {Randal J.} and Chang, {Annie C.Y.} and Cohen, {Stanley N.} and Schimke, {Robert T.}",

note = "Funding Information: This work was supported by research grants from the NIH and the American Cancer Society.",

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T1 - Structure and genomic organization of the mouse dihydrofolate reductase gene

AU - Nunberg, Jack H.

AU - Kaufman, Randal J.

AU - Chang, Annie C.Y.

AU - Cohen, Stanley N.

AU - Schimke, Robert T.

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N2 - The genomic organization of the mouse dihydrofolate reductase gene has been determined by hybridization of specific cDNA sequences to restriction endonuclease-generated fragments of DNA from methotrexate-resistant S-180 cells. The dihydrofolate reductase gene contains a minimum of five intervening sequences (one in the 5′ untranslated region and four in the protein-coding region) and spans a minimum of 42 kilobase pairs on the genome. Genomic sequences at the junction of the intervening sequence and mRNA-coding sequence and at the polyadenylation site have been determined. A similar organization is found in independently isolated methotrexate-resistant cell lines, in the parental sensitive cell line and in several inbred mouse strains, indicating that this organization represents that of the natural gene.

AB - The genomic organization of the mouse dihydrofolate reductase gene has been determined by hybridization of specific cDNA sequences to restriction endonuclease-generated fragments of DNA from methotrexate-resistant S-180 cells. The dihydrofolate reductase gene contains a minimum of five intervening sequences (one in the 5′ untranslated region and four in the protein-coding region) and spans a minimum of 42 kilobase pairs on the genome. Genomic sequences at the junction of the intervening sequence and mRNA-coding sequence and at the polyadenylation site have been determined. A similar organization is found in independently isolated methotrexate-resistant cell lines, in the parental sensitive cell line and in several inbred mouse strains, indicating that this organization represents that of the natural gene.

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Structure and genomic organization of the mouse dihydrofolate reductase gene

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