Structure and genomic organization of the mouse dihydrofolate reductase gene

Jack H. Nunberg, Randal J. Kaufman, Annie C.Y. Chang, Stanley N. Cohen, Robert T. Schimke

Research output: Contribution to journalArticlepeer-review

Abstract

The genomic organization of the mouse dihydrofolate reductase gene has been determined by hybridization of specific cDNA sequences to restriction endonuclease-generated fragments of DNA from methotrexate-resistant S-180 cells. The dihydrofolate reductase gene contains a minimum of five intervening sequences (one in the 5′ untranslated region and four in the protein-coding region) and spans a minimum of 42 kilobase pairs on the genome. Genomic sequences at the junction of the intervening sequence and mRNA-coding sequence and at the polyadenylation site have been determined. A similar organization is found in independently isolated methotrexate-resistant cell lines, in the parental sensitive cell line and in several inbred mouse strains, indicating that this organization represents that of the natural gene.

Original languageEnglish
Pages (from-to)355-364
Number of pages10
JournalCell
Volume19
Issue number2
DOIs
StatePublished - Feb 1980

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