Structure and genomic organization of the mouse dihydrofolate reductase gene

Jack H. Nunberg; Randal J. Kaufman; Annie C.Y. Chang; Stanley N. Cohen; Robert T. Schimke

doi:10.1016/0092-8674(80)90510-3

Structure and genomic organization of the mouse dihydrofolate reductase gene

Jack H. Nunberg
, Randal J. Kaufman
, Annie C.Y. Chang
, Stanley N. Cohen
, Robert T. Schimke

Montana Biotechnology Center

Stanford University

Research output: Contribution to journal › Article › peer-review

107 Scopus citations

Abstract

The genomic organization of the mouse dihydrofolate reductase gene has been determined by hybridization of specific cDNA sequences to restriction endonuclease-generated fragments of DNA from methotrexate-resistant S-180 cells. The dihydrofolate reductase gene contains a minimum of five intervening sequences (one in the 5′ untranslated region and four in the protein-coding region) and spans a minimum of 42 kilobase pairs on the genome. Genomic sequences at the junction of the intervening sequence and mRNA-coding sequence and at the polyadenylation site have been determined. A similar organization is found in independently isolated methotrexate-resistant cell lines, in the parental sensitive cell line and in several inbred mouse strains, indicating that this organization represents that of the natural gene.

Original language	English
Pages (from-to)	355-364
Number of pages	10
Journal	Cell
Volume	19
Issue number	2
DOIs	https://doi.org/10.1016/0092-8674(80)90510-3
State	Published - Feb 1980

Funding

This work was supported by research grants from the NIH and the American Cancer Society.

Funders
American Cancer Society

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10.1016/0092-8674(80)90510-3

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title = "Structure and genomic organization of the mouse dihydrofolate reductase gene",

abstract = "The genomic organization of the mouse dihydrofolate reductase gene has been determined by hybridization of specific cDNA sequences to restriction endonuclease-generated fragments of DNA from methotrexate-resistant S-180 cells. The dihydrofolate reductase gene contains a minimum of five intervening sequences (one in the 5′ untranslated region and four in the protein-coding region) and spans a minimum of 42 kilobase pairs on the genome. Genomic sequences at the junction of the intervening sequence and mRNA-coding sequence and at the polyadenylation site have been determined. A similar organization is found in independently isolated methotrexate-resistant cell lines, in the parental sensitive cell line and in several inbred mouse strains, indicating that this organization represents that of the natural gene.",

author = "Nunberg, \{Jack H.\} and Kaufman, \{Randal J.\} and Chang, \{Annie C.Y.\} and Cohen, \{Stanley N.\} and Schimke, \{Robert T.\}",

note = "Funding Information: This work was supported by research grants from the NIH and the American Cancer Society.",

year = "1980",

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doi = "10.1016/0092-8674(80)90510-3",

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T1 - Structure and genomic organization of the mouse dihydrofolate reductase gene

AU - Nunberg, Jack H.

AU - Kaufman, Randal J.

AU - Chang, Annie C.Y.

AU - Cohen, Stanley N.

AU - Schimke, Robert T.

N1 - Funding Information: This work was supported by research grants from the NIH and the American Cancer Society.

PY - 1980/2

Y1 - 1980/2

N2 - The genomic organization of the mouse dihydrofolate reductase gene has been determined by hybridization of specific cDNA sequences to restriction endonuclease-generated fragments of DNA from methotrexate-resistant S-180 cells. The dihydrofolate reductase gene contains a minimum of five intervening sequences (one in the 5′ untranslated region and four in the protein-coding region) and spans a minimum of 42 kilobase pairs on the genome. Genomic sequences at the junction of the intervening sequence and mRNA-coding sequence and at the polyadenylation site have been determined. A similar organization is found in independently isolated methotrexate-resistant cell lines, in the parental sensitive cell line and in several inbred mouse strains, indicating that this organization represents that of the natural gene.

AB - The genomic organization of the mouse dihydrofolate reductase gene has been determined by hybridization of specific cDNA sequences to restriction endonuclease-generated fragments of DNA from methotrexate-resistant S-180 cells. The dihydrofolate reductase gene contains a minimum of five intervening sequences (one in the 5′ untranslated region and four in the protein-coding region) and spans a minimum of 42 kilobase pairs on the genome. Genomic sequences at the junction of the intervening sequence and mRNA-coding sequence and at the polyadenylation site have been determined. A similar organization is found in independently isolated methotrexate-resistant cell lines, in the parental sensitive cell line and in several inbred mouse strains, indicating that this organization represents that of the natural gene.

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DO - 10.1016/0092-8674(80)90510-3

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VL - 19

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ER -

Structure and genomic organization of the mouse dihydrofolate reductase gene

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