Synthesis, characterization and DNA binding affinities of water-soluble benzoheterocycle triosmium clusters

E. Rosenberg; F. Spada; K. Sugden; B. Martin; Luciano Milone; Roberto Gobetto; Alessandra Viale; Jan Fiedler

doi:10.1016/S0022-328X(02)02097-1

Synthesis, characterization and DNA binding affinities of water-soluble benzoheterocycle triosmium clusters

E. Rosenberg
, F. Spada
, K. Sugden
, B. Martin
, Luciano Milone
, Roberto Gobetto
, Alessandra Viale
, Jan Fiedler

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

The syntheses of the water-soluble clusters [Os ₃ (CO) ₉ (μ-η ² - (L-H))(μ-H)L′] (L=3-amino quinoline, L′=Na ₃ [P(C ₆ H ₄ SO ₃ ) ₃ ] 1; L=3-amino quinoline, L′=[P(OCH ₂ CH ₂ NMe ₃ ) ₃ ]I ₃ , 2; L=3-(2-phenyl acetimido) quinoline, L′=[P(OCH ₂ CH ₂ NMe ₃ ) ₃ ]I ₃ , 3; L= phenanthridine, L′=[P(OCH ₂ CH ₂ NMe ₃ ) ₃ ]I ₃ , 4) are reported. The products have been fully characterized by ¹ H-, ¹³ C VT-NMR and LC-TOF-MS. The effect of pH and concentration on intermolecular aggregation in water has been investigated. The interactions of these clusters with DNA have been studied using the plasmid super coiled DNA relaxation test in a 1% electrophoresis agarose gel. Band retardation due to cluster binding was observed for the positively charged clusters 2-4, but not for the negatively charged 1. The relative binding affinities were in the order 2<3∼4 and all three showed greater binding affinities than the positively charged cluster [Rh ₃ (μ ₃ -S) ₂ (η ⁵ - Me ₅ C ₅ ) ₃ ](BF ₄ ) ₂ . These preliminary results suggest a relationship between the nature of the heterocyclic ligand and the binding affinity of the cluster to DNA.

Original language	English
Pages (from-to)	51-58
Number of pages	8
Journal	Journal of Organometallic Chemistry
Volume	668
Issue number	1-2
DOIs	https://doi.org/10.1016/S0022-328X(02)02097-1
State	Published - Feb 17 2003

Funding

We thank the NATO Collaborative Science Grant Program, the Montana NSF EPSCoR Competitive Grant Program, the University of Montana and the Università di Torino for support of this research.

Keywords

DNA
Metal clusters
Osmium
Water soluble

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10.1016/S0022-328X(02)02097-1

Cite this

@article{7bbdeab98d604c098cf4d4f61ca894c3,

title = "Synthesis, characterization and DNA binding affinities of water-soluble benzoheterocycle triosmium clusters",

abstract = " The syntheses of the water-soluble clusters [Os 3 (CO) 9 (μ-η 2 - (L-H))(μ-H)L′] (L=3-amino quinoline, L′=Na 3 [P(C 6 H 4 SO 3 ) 3 ] 1; L=3-amino quinoline, L′=[P(OCH 2 CH 2 NMe 3 ) 3 ]I 3 , 2; L=3-(2-phenyl acetimido) quinoline, L′=[P(OCH 2 CH 2 NMe 3 ) 3 ]I 3 , 3; L= phenanthridine, L′=[P(OCH 2 CH 2 NMe 3 ) 3 ]I 3 , 4) are reported. The products have been fully characterized by 1 H-, 13 C VT-NMR and LC-TOF-MS. The effect of pH and concentration on intermolecular aggregation in water has been investigated. The interactions of these clusters with DNA have been studied using the plasmid super coiled DNA relaxation test in a 1\% electrophoresis agarose gel. Band retardation due to cluster binding was observed for the positively charged clusters 2-4, but not for the negatively charged 1. The relative binding affinities were in the order 2<3∼4 and all three showed greater binding affinities than the positively charged cluster [Rh 3 (μ 3 -S) 2 (η 5 - Me 5 C 5 ) 3 ](BF 4 ) 2 . These preliminary results suggest a relationship between the nature of the heterocyclic ligand and the binding affinity of the cluster to DNA.",

keywords = "DNA, Metal clusters, Osmium, Water soluble",

author = "E. Rosenberg and F. Spada and K. Sugden and B. Martin and Luciano Milone and Roberto Gobetto and Alessandra Viale and Jan Fiedler",

note = "Funding Information: We thank the NATO Collaborative Science Grant Program, the Montana NSF EPSCoR Competitive Grant Program, the University of Montana and the Universit{\`a} di Torino for support of this research.",

year = "2003",

month = feb,

day = "17",

doi = "10.1016/S0022-328X(02)02097-1",

language = "English",

volume = "668",

pages = "51--58",

journal = "Journal of Organometallic Chemistry",

issn = "0022-328X",

publisher = "Elsevier B.V.",

number = "1-2",

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TY - JOUR

T1 - Synthesis, characterization and DNA binding affinities of water-soluble benzoheterocycle triosmium clusters

AU - Rosenberg, E.

AU - Spada, F.

AU - Sugden, K.

AU - Martin, B.

AU - Milone, Luciano

AU - Gobetto, Roberto

AU - Viale, Alessandra

AU - Fiedler, Jan

N1 - Funding Information: We thank the NATO Collaborative Science Grant Program, the Montana NSF EPSCoR Competitive Grant Program, the University of Montana and the Università di Torino for support of this research.

PY - 2003/2/17

Y1 - 2003/2/17

N2 - The syntheses of the water-soluble clusters [Os 3 (CO) 9 (μ-η 2 - (L-H))(μ-H)L′] (L=3-amino quinoline, L′=Na 3 [P(C 6 H 4 SO 3 ) 3 ] 1; L=3-amino quinoline, L′=[P(OCH 2 CH 2 NMe 3 ) 3 ]I 3 , 2; L=3-(2-phenyl acetimido) quinoline, L′=[P(OCH 2 CH 2 NMe 3 ) 3 ]I 3 , 3; L= phenanthridine, L′=[P(OCH 2 CH 2 NMe 3 ) 3 ]I 3 , 4) are reported. The products have been fully characterized by 1 H-, 13 C VT-NMR and LC-TOF-MS. The effect of pH and concentration on intermolecular aggregation in water has been investigated. The interactions of these clusters with DNA have been studied using the plasmid super coiled DNA relaxation test in a 1% electrophoresis agarose gel. Band retardation due to cluster binding was observed for the positively charged clusters 2-4, but not for the negatively charged 1. The relative binding affinities were in the order 2<3∼4 and all three showed greater binding affinities than the positively charged cluster [Rh 3 (μ 3 -S) 2 (η 5 - Me 5 C 5 ) 3 ](BF 4 ) 2 . These preliminary results suggest a relationship between the nature of the heterocyclic ligand and the binding affinity of the cluster to DNA.

AB - The syntheses of the water-soluble clusters [Os 3 (CO) 9 (μ-η 2 - (L-H))(μ-H)L′] (L=3-amino quinoline, L′=Na 3 [P(C 6 H 4 SO 3 ) 3 ] 1; L=3-amino quinoline, L′=[P(OCH 2 CH 2 NMe 3 ) 3 ]I 3 , 2; L=3-(2-phenyl acetimido) quinoline, L′=[P(OCH 2 CH 2 NMe 3 ) 3 ]I 3 , 3; L= phenanthridine, L′=[P(OCH 2 CH 2 NMe 3 ) 3 ]I 3 , 4) are reported. The products have been fully characterized by 1 H-, 13 C VT-NMR and LC-TOF-MS. The effect of pH and concentration on intermolecular aggregation in water has been investigated. The interactions of these clusters with DNA have been studied using the plasmid super coiled DNA relaxation test in a 1% electrophoresis agarose gel. Band retardation due to cluster binding was observed for the positively charged clusters 2-4, but not for the negatively charged 1. The relative binding affinities were in the order 2<3∼4 and all three showed greater binding affinities than the positively charged cluster [Rh 3 (μ 3 -S) 2 (η 5 - Me 5 C 5 ) 3 ](BF 4 ) 2 . These preliminary results suggest a relationship between the nature of the heterocyclic ligand and the binding affinity of the cluster to DNA.

KW - DNA

KW - Metal clusters

KW - Osmium

KW - Water soluble

UR - https://www.scopus.com/pages/publications/0037450684

U2 - 10.1016/S0022-328X(02)02097-1

DO - 10.1016/S0022-328X(02)02097-1

M3 - Article

AN - SCOPUS:0037450684

SN - 0022-328X

VL - 668

SP - 51

EP - 58

JO - Journal of Organometallic Chemistry

JF - Journal of Organometallic Chemistry

IS - 1-2

ER -

Synthesis, characterization and DNA binding affinities of water-soluble benzoheterocycle triosmium clusters

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Keywords

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