Targeted retrieval and analysis of five neandertal mtDNA genomes

Adrian W. Briggs; Jeffrey M. Good; Richard E. Green; Johannes Krause; Tomislav Maricic; Udo Stenzel; Carles Lalueza-Fox; Pavao Rudan; Dejana Brajković; Zeljko Kućan; Ivan Gušić; Ralf Schmitz; Vladimir B. Doronichev; Liubov V. Golovanova; Marco De La Rasilla; Javier Fortea; Antonio Rosas; Svante Pääbo

doi:10.1126/science.1174462

Targeted retrieval and analysis of five neandertal mtDNA genomes

Adrian W. Briggs, Jeffrey M. Good, Richard E. Green, Johannes Krause, Tomislav Maricic, Udo Stenzel, Carles Lalueza-Fox, Pavao Rudan, Dejana Brajković, Zeljko Kućan, Ivan Gušić, Ralf Schmitz, Vladimir B. Doronichev, Liubov V. Golovanova, Marco De La Rasilla, Javier Fortea, Antonio Rosas, Svante Pääbo

Biological Sciences

Research output: Contribution to journal › Article › peer-review

392 Scopus citations

Abstract

Analysis of Neandertal DNA holds great potential for investigating the population history of this group of hominins, but progress has been limited due to the rarity of samples and damaged state of the DNA. We present a method of targeted ancient DNA sequence retrieval that greatly reduces sample destruction and sequencing demands and use this method to reconstruct the complete mitochondrial DNA (mtDNA) genomes of five Neandertals from across their geographic range. We find that mtDNA genetic diversity in Neandertals that lived 38,000 to 70,000 years ago was approximately one-third of that in contemporary modern humans. Together with analyses of mtDNA protein evolution, these data suggest that the long-term effective population size of Neandertals was smaller than that of modern humans and extant great apes.

Original language	English
Pages (from-to)	318-321
Number of pages	4
Journal	Science
Volume	325
Issue number	5938
DOIs	https://doi.org/10.1126/science.1174462
State	Published - Jul 17 2009

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Briggs, A. W., Good, J. M., Green, R. E., Krause, J., Maricic, T., Stenzel, U., Lalueza-Fox, C., Rudan, P., Brajković, D., Kućan, Z., Gušić, I., Schmitz, R., Doronichev, V. B., Golovanova, L. V., De La Rasilla, M., Fortea, J., Rosas, A., & Pääbo, S. (2009). Targeted retrieval and analysis of five neandertal mtDNA genomes. Science, 325(5938), 318-321. https://doi.org/10.1126/science.1174462

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title = "Targeted retrieval and analysis of five neandertal mtDNA genomes",

abstract = "Analysis of Neandertal DNA holds great potential for investigating the population history of this group of hominins, but progress has been limited due to the rarity of samples and damaged state of the DNA. We present a method of targeted ancient DNA sequence retrieval that greatly reduces sample destruction and sequencing demands and use this method to reconstruct the complete mitochondrial DNA (mtDNA) genomes of five Neandertals from across their geographic range. We find that mtDNA genetic diversity in Neandertals that lived 38,000 to 70,000 years ago was approximately one-third of that in contemporary modern humans. Together with analyses of mtDNA protein evolution, these data suggest that the long-term effective population size of Neandertals was smaller than that of modern humans and extant great apes.",

author = "Briggs, {Adrian W.} and Good, {Jeffrey M.} and Green, {Richard E.} and Johannes Krause and Tomislav Maricic and Udo Stenzel and Carles Lalueza-Fox and Pavao Rudan and Dejana Brajkovi{\'c} and Zeljko Ku{\'c}an and Ivan Gu{\v s}i{\'c} and Ralf Schmitz and Doronichev, {Vladimir B.} and Golovanova, {Liubov V.} and {De La Rasilla}, Marco and Javier Fortea and Antonio Rosas and Svante P{\"a}{\"a}bo",

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Briggs, AW, Good, JM, Green, RE, Krause, J, Maricic, T, Stenzel, U, Lalueza-Fox, C, Rudan, P, Brajković, D, Kućan, Z, Gušić, I, Schmitz, R, Doronichev, VB, Golovanova, LV, De La Rasilla, M, Fortea, J, Rosas, A & Pääbo, S 2009, 'Targeted retrieval and analysis of five neandertal mtDNA genomes', Science, vol. 325, no. 5938, pp. 318-321. https://doi.org/10.1126/science.1174462

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T1 - Targeted retrieval and analysis of five neandertal mtDNA genomes

AU - Briggs, Adrian W.

AU - Good, Jeffrey M.

AU - Green, Richard E.

AU - Krause, Johannes

AU - Maricic, Tomislav

AU - Stenzel, Udo

AU - Lalueza-Fox, Carles

AU - Rudan, Pavao

AU - Brajković, Dejana

AU - Kućan, Zeljko

AU - Gušić, Ivan

AU - Schmitz, Ralf

AU - Doronichev, Vladimir B.

AU - Golovanova, Liubov V.

AU - De La Rasilla, Marco

AU - Fortea, Javier

AU - Rosas, Antonio

AU - Pääbo, Svante

PY - 2009/7/17

Y1 - 2009/7/17

N2 - Analysis of Neandertal DNA holds great potential for investigating the population history of this group of hominins, but progress has been limited due to the rarity of samples and damaged state of the DNA. We present a method of targeted ancient DNA sequence retrieval that greatly reduces sample destruction and sequencing demands and use this method to reconstruct the complete mitochondrial DNA (mtDNA) genomes of five Neandertals from across their geographic range. We find that mtDNA genetic diversity in Neandertals that lived 38,000 to 70,000 years ago was approximately one-third of that in contemporary modern humans. Together with analyses of mtDNA protein evolution, these data suggest that the long-term effective population size of Neandertals was smaller than that of modern humans and extant great apes.

AB - Analysis of Neandertal DNA holds great potential for investigating the population history of this group of hominins, but progress has been limited due to the rarity of samples and damaged state of the DNA. We present a method of targeted ancient DNA sequence retrieval that greatly reduces sample destruction and sequencing demands and use this method to reconstruct the complete mitochondrial DNA (mtDNA) genomes of five Neandertals from across their geographic range. We find that mtDNA genetic diversity in Neandertals that lived 38,000 to 70,000 years ago was approximately one-third of that in contemporary modern humans. Together with analyses of mtDNA protein evolution, these data suggest that the long-term effective population size of Neandertals was smaller than that of modern humans and extant great apes.

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SN - 0036-8075

VL - 325

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Targeted retrieval and analysis of five neandertal mtDNA genomes

Abstract

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