Targeting of Arf-1 to the early Golgi by membrin, an ER-Golgi SNARE

Akira Honda, Omayma S. Al-Awar, Jesse C. Hay, Julie G. Donaldson

Research output: Contribution to journalArticlepeer-review

75 Scopus citations


Arf and Rab family GTPases regulate membrane traffic in cells, yet little is known about how they are targeted to distinct organelles. To identify sequences in Arf-1 necessary for Golgi targeting, we examined the localization of chimeras between Arf-1 and Arf-6. Here, we identify a 16-amino acid sequence in Arf-1 that specifies Golgi targeting and contains a motif (MXXE) that is important for Arf-1 binding to membrin, an ER-Golgi SNARE protein. The MXXE motif is conserved in all Arfs known to localize to the Golgi and enables Arf-1 to localize to the early Golgi. Arf-1 lacking these 16 aa can still localize to the late Golgi where it displays a more rapid Golgi-cytosol cycle than wild-type Arf-1. These studies suggest that membrin recruits Arf-1 to the early Golgi and reveal distinct kinetic cycles for Arf-1 at early and late Golgi determined by different sets of Arf regulators and effectors.

Original languageEnglish
Pages (from-to)1039-1051
Number of pages13
JournalJournal of Cell Biology
Issue number7
StatePublished - Mar 28 2005


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