@article{44f43954f4ed4e7d8517f66c839ec55a,
title = "Taxol formation in yew - Taxus",
abstract = "In Pacific yew, Taxus brevifolia, taxol is found most abundantly in the vascular cambial region followed by the phloem, sapwood and heartwood. It is in this order that 'in vitro' taxol biosynthetic activity was also demonstrated using [1-14C]acetate as a precursor. However, only traces of taxol biosynthesis could be demonstrated in the xylem (sapwood-heartwood) suggesting that taxol may be mobilized from its place of greatest biosynthetic activity (vascular cambial region) to the xylem; perhaps via ray parenchyma. Taxol could also be effectively trapped under 'bark flaps', over a period of several weeks, using silica gel powder as an adsorbant. In vitro taxol biosynthesis using [1-14C]acetate in outer bark samples (cambium-phloem) could only be demonstrated in Taxus brevifolia, Taxus floridana and Taxus canadensis, among 10 of the 11 native Taxus species tested.",
keywords = "Cambial area, Cancer, Phloem, Taxus spp, Xylem, [C]Taxol",
author = "Strobel, {Gary A.} and Andrea Stierle and Hess, {W. M.}",
note = "Funding Information: China, and his brother-in-law most graciously sacrificed time and effort to acquire and send samples of T. mairei. 7{"}. brevifolia from the State of Washington was supplied by Drs. Heinz Floss and Ursula Mocek. Dr. David Bailey and colleagues of Hauser, Co., Boulder, Colorado, provided assistance in quantitating taxol in some of our samples. The authors also acknowledge Suzan Strobel's help in collecting samples of T. brevifolia in Montana and doing the yew log drawing (Fig. 6). Dr. Scott Strobel is thanked for his drawing of the taxol structure. Authentic samples of taxol were provided by both the National Cancer Institute and Bristol Myers Squibb Co. Mr. A1 Christoph-ersen, District Ranger on the Flathead National Forest, Montana, provided advice and permits for Taxus collection. Mr. Dennis Bodily of Bozeman, Montana, assisted in collecting yew samples and in preparing samples for examination. Financial support from the National Science Foundation, National Cancer Institute, the Montana Science and Technology Alliance, the American Cancer Society, and the Montana Agricultural Experiment Station is also appreciated.",
year = "1993",
doi = "10.1016/0168-9452(93)90060-D",
language = "English",
volume = "92",
pages = "1--12",
journal = "Plant Science",
issn = "0168-9452",
publisher = "Elsevier Ireland Ltd",
number = "1",
}
