TY - JOUR
T1 - The effects of acute exercise on neutrophils and plasma oxidative stress
AU - Quindry, John C.
AU - Stone, William L.
AU - King, Jeff
AU - Broeder, Craig E.
PY - 2003/7/1
Y1 - 2003/7/1
N2 - Purpose: To investigate the influence of intensity versus total energy expenditure on neutrophilia and blood oxidative stress to acute exercise. Methods: Nine males (18-30 yr) completed one maximal (Max) and three submaximal exercise sessions: 1) 45 min at 10% above (LT+) lactate threshold (LT), 2) 45 min at 10% below (LT-) LT, and 3) 10% below LT until caloric expenditure equaled the 10%+ trial (LT-kcal). Blood was sampled before (PRE), immediately (POST), 1 h, and 2 h after exercise to measure neutrophils, myeloperoxidase, superoxide (O2-), neutrophil activation (O2-/neutrophils), ascorbic acid, uric acid, malondialdehyde, and lipid hydroperoxides. Results: Intensity-dependent neutrophilia occurred POST exercise with significant increases (P ≤ 0.05) after Max and LT+. A second neutrophilia wave occurred 2 h postexercise. Superoxide was elevated POST (Max) and 2 h post (Max and LT+). In contrast, O2-/neutrophils was increased at 2 h only (Max and LT +). These data indicate that immediately postexercise, total neutrophil number rather than activation best represents neutrophil-generated reactive species within blood. POST Max, ascorbic acid and uric acid were decreased indicating a blood oxidative stress occurred. Alternately, total energy expenditure was not related to any marker of neutrophilia or oxidative stress. Conclusion: Exercise intensity plays a major role in postexercise blood oxidative stress, whereas total exercise energy expenditure does not. Further, neutrophils recruited into circulation during exercise may impose a threshold dependent oxidative stress in blood plasma after exercise.
AB - Purpose: To investigate the influence of intensity versus total energy expenditure on neutrophilia and blood oxidative stress to acute exercise. Methods: Nine males (18-30 yr) completed one maximal (Max) and three submaximal exercise sessions: 1) 45 min at 10% above (LT+) lactate threshold (LT), 2) 45 min at 10% below (LT-) LT, and 3) 10% below LT until caloric expenditure equaled the 10%+ trial (LT-kcal). Blood was sampled before (PRE), immediately (POST), 1 h, and 2 h after exercise to measure neutrophils, myeloperoxidase, superoxide (O2-), neutrophil activation (O2-/neutrophils), ascorbic acid, uric acid, malondialdehyde, and lipid hydroperoxides. Results: Intensity-dependent neutrophilia occurred POST exercise with significant increases (P ≤ 0.05) after Max and LT+. A second neutrophilia wave occurred 2 h postexercise. Superoxide was elevated POST (Max) and 2 h post (Max and LT+). In contrast, O2-/neutrophils was increased at 2 h only (Max and LT +). These data indicate that immediately postexercise, total neutrophil number rather than activation best represents neutrophil-generated reactive species within blood. POST Max, ascorbic acid and uric acid were decreased indicating a blood oxidative stress occurred. Alternately, total energy expenditure was not related to any marker of neutrophilia or oxidative stress. Conclusion: Exercise intensity plays a major role in postexercise blood oxidative stress, whereas total exercise energy expenditure does not. Further, neutrophils recruited into circulation during exercise may impose a threshold dependent oxidative stress in blood plasma after exercise.
KW - Antioxidant
KW - Lipid hyroperoxide
KW - Myeloperoxidase
KW - Superoxide
KW - White blood cell
UR - http://www.scopus.com/inward/record.url?scp=0038693133&partnerID=8YFLogxK
U2 - 10.1249/01.MSS.0000074568.82597.0B
DO - 10.1249/01.MSS.0000074568.82597.0B
M3 - Article
C2 - 12840634
AN - SCOPUS:0038693133
SN - 0195-9131
VL - 35
SP - 1139
EP - 1145
JO - Medicine and Science in Sports and Exercise
JF - Medicine and Science in Sports and Exercise
IS - 7
ER -