@article{80385b43dadb4af98266efe46df80b46,
title = "The lysophospholipid acyltransferase antagonist CI-976 inhibits a late step in COPII vesicle budding",
abstract = "The mechanism of coat protein (COP)II vesicle fission from the endoplasmic reticulum (ER) remains unclear. Lysophospholipid acyltransferases (LPATs) catalyze the conversion of various lysophospholipids to phospholipids, a process that can promote spontaneous changes in membrane curvature. Here, we show that 2,2-methyl-N-(2,4,6,-trimethoxyphenyl) dodecanamide (CI-976), a potent LPAT inhibitor, reversibly inhibited export from the ER in vivo and the formation of COPII vesicles in vitro. Moreover, CI-976 caused the rapid and reversible accumulation of cargo at ER exit sites (ERESs) containing the COPII coat components Sec23/24 and Sec13/31 and a marked enhancement of Sar1p-mediated tubule formation from ERESs, suggesting that CI-976 inhibits the fission of assembled COPII budding elements. These results identify a small molecule inhibitor of a very late step in COPII vesicle formation, consistent with fission inhibition, and demonstrate that this step is likely facilitated by an ER-associated LPAT.",
keywords = "COPII vesicles, ER exit sites, Lysophospholipid acyltransferase, Membrane curvature, Vesicle fission",
author = "Brown, \{William J.\} and Helen Plutner and Daniel Drecktrah and Judson, \{Bret L.\} and Balch, \{William E.\}",
year = "2008",
month = may,
doi = "10.1111/j.1600-0854.2008.00711.x",
language = "English",
volume = "9",
pages = "786--797",
journal = "Traffic",
issn = "1398-9219",
publisher = "Blackwell Munksgaard",
number = "5",
}