The missing linker: A dimerization motif located within polyketide synthase modules

Jianting Zheng, Christopher D. Fage, Borries Demeler, David W. Hoffman, Adrian T. Keatinge-Clay

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


The dimerization of multimodular polyketide synthases is essential for their function. Motifs that supplement the contacts made by dimeric polyketide synthase enzymes have previously been characterized outside the boundaries of modules, at the N- and C-terminal ends of polyketide synthase subunits. Here we describe a heretofore uncharacterized dimerization motif located within modules. The dimeric state of this dimerization element was elucidated through the 2.6 Å resolution crystal structure of a fragment containing a dimerization element and a ketoreductase. The solution structure of a standalone dimerization element was revealed by nuclear magnetic resonance spectroscopy to be consistent with that of the crystal structure, and its dimerization constant was measured through analytical ultracentrifugation to be ∼20 μM. The dimer buries ∼990 Å2 at its interface, and its C-terminal helices rigidly connect to ketoreductase domains to constrain their locations within a module. These structural restraints permitted the construction of a common type of polyketide synthase module.

Original languageEnglish
Pages (from-to)1263-1270
Number of pages8
JournalACS Chemical Biology
Issue number6
StatePublished - Jun 21 2013


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