Abstract
Most animals can distinguish two distinct types of touch stimuli: gentle (innocuous) and harsh (noxious/painful) touch, however, the underlying mechanisms are not well understood. Caenorhabditis elegans is a useful model for the study of gentle touch sensation. However, little is known about harsh touch sensation in this organism. Here we characterize harsh touch sensation in C. elegans. We show that C. elegans exhibits differential behavioural responses to harsh touch and gentle touch. Laser ablations identify distinct sets of sensory neurons and interneurons required for harsh touch sensation at different body segments. Optogenetic stimulation of the circuitry can drive behaviour. Patch-clamp recordings reveal that TRP family and amiloride-sensitive Na + channels mediate touch-evoked currents in different sensory neurons. Our work identifies the neural circuits and characterizes the sensory channels mediating harsh touch sensation in C. elegans, establishing it as a genetic model for studying this sensory modality.
| Original language | English |
|---|---|
| Article number | 315 |
| Journal | Nature Communications |
| Volume | 2 |
| Issue number | 1 |
| DOIs | |
| State | Published - 2011 |
Funding
We thank A. Kumar for comments, L. Looger for G-CaMP3.0, W. Schafer for strains and A. Gottschalk for ChR2 plasmid. Some strains were obtained from the Caenorhabditis Genetics Center and Knockout Consortiums in the USA and Japan. This work was supported by an American Heart Association postdoctoral fellowship (W.L.) and grants from the NIGMS (X.Z.S.X.) and Pew Scholars Program (X.Z.S.X).
| Funders | Funder number |
|---|---|
| R01GM083241 | |
| American Heart Association |