The P granule component PGL-1 promotes the localization and silencing activity of the PUF protein FBF-2 in germline stem cells

Ekaterina Voronina, Alexandre Paix, Geraldine Seydoux

Research output: Contribution to journalArticlepeer-review

Abstract

In the C. elegans germline, maintenance of undifferentiated stem cells depends on the PUF family RNA-binding proteins FBF-1 and FBF-2. FBF-1 and FBF-2 are 89% identical and are required redundantly to silence the expression of mRNAs that promote meiosis. Here we show that, despite their extensive sequence similarity, FBF-1 and FBF-2 have different effects on target mRNAs. FBF-1 promotes the degradation and/or transport of meiotic mRNAs out of the stem cell region, whereas FBF-2 prevents translation. FBF-2 activity depends on the P granule component PGL-1. PGL-1 is required to localize FBF-2 to perinuclear P granules and for efficient binding of FBF-2 to its mRNA targets. We conclude that multiple regulatory mechanisms converge on meiotic RNAs to ensure silencing in germline stem cells. Our findings also support the view that P granules facilitate mRNA silencing by providing an environment in which translational repressors can encounter their mRNA targets immediately upon exit from the nucleus.

Original languageEnglish
Pages (from-to)3732-3740
Number of pages9
JournalDevelopment (Cambridge)
Volume139
Issue number20
DOIs
StatePublished - Oct 15 2012

Keywords

  • Caenorhabditis elegans
  • Germline stem cells
  • P granules
  • PGL-1
  • PUF domain
  • RNA silencing

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