Abstract
We have shown that chlorocholine chloride (CCC), succinic acid, 2,2-dimethylhydrazide (Alar®) and tetramethylammonium bromide (TMAB) stimulate [14C]acetate incorporation into taxol in intact pieces of the inner bark of Taxus brevifolia (Pacific yew). Both CCC and Alar also stimulated taxol production in yew logs as measured by the incorporation of [1-14C]acetate into [14C]taxol. An increase in taxol accumulation also occured in silica gel placed under flaps of yew trees in the forest treated with these two compounds. CCC also caused an increase, in an 8-week treatment period, of recoverable taxol from Pacific yew bark in a forest setting. CCC, alar and TMAB are all known as growth retardants, but may cause an effect on taxol biosynthesis via an inhibition of sterol biosynthesis.
| Original language | English |
|---|---|
| Pages (from-to) | 115-124 |
| Number of pages | 10 |
| Journal | Plant Science |
| Volume | 101 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1994 |
Funding
The authors appreciatet he drawing of the yew log provided by Suzan Strobel. Alar was a generousg ift of the Uniroyal Chemical Company Inc. The supporta nd help of the peopleo f the U.S. Forest Service, Hungry Horse District, Flathead National Forest in acknowledgedT.h e Yellow Bay Laboratory of the University of Montana has generouslyp rovideda ccommodationdsu ring our trips to Northern Montana. Taxol and other tax-anesa re a gift of the NC1. Some taxol and taxane analysesin this report were independentldyo neby Hauser Chemical Company, Boulder, CO, com-plimentso f Dr David Bailey. Financial supportf or this project has come from the Montana Science and TechnologyA lliance, the Montana Agricultural ExperimentS tation,a nd the National Cancer Institute, and Cytoclonal Company, Dallas, Texas.
Keywords
- Alar
- Chlorocholine chloride
- Growth retardants
- Sterol synthesis inhibitors
- Taxol
- Taxus spp.