TY - JOUR
T1 - Transferrin receptor 1 is a cellular receptor for New World haemorrhagic fever arenaviruses
AU - Radoshitzky, Sheli R.
AU - Abraham, Jonathan
AU - Spiropoulou, Christina F.
AU - Kuhn, Jens H.
AU - Nguyen, Dan
AU - Li, Wenhui
AU - Nagel, Jane
AU - Schmidt, Paul J.
AU - Nunberg, Jack H.
AU - Andrews, Nancy C.
AU - Farzan, Michael
AU - Choe, Hyeryun
PY - 2007/3/1
Y1 - 2007/3/1
N2 - At least five arenaviruses cause viral haemorrhagic fevers in humans. Lassa virus, an Old World arenavirus, uses the cellular receptor α-dystroglycan to infect cells. Machupo, Guanarito, Junin and Sabia viruses are New World haemorrhagic fever viruses that do not use α-dystroglycan. Here we show a specific, high-affinity association between transferrin receptor 1 (TfR1) and the entry glycoprotein (GP) of Machupo virus. Expression of human TfR1, but not human transferrin receptor 2, in hamster cell lines markedly enhanced the infection of viruses pseudotyped with the GP of Machupo, Guanarito and Junin viruses, but not with those of Lassa or lymphocytic choriomeningitis viruses. An anti-TfR1 antibody efficiently inhibited the replication of Machupo, Guanarito, Junin and Sabia viruses, but not that of Lassa virus. Iron depletion of culture medium enhanced, and iron supplementation decreased, the efficiency of infection by Junin and Machupo but not Lassa pseudoviruses. These data indicate that TfR1 is a cellular receptor for New World haemorrhagic fever arenaviruses.
AB - At least five arenaviruses cause viral haemorrhagic fevers in humans. Lassa virus, an Old World arenavirus, uses the cellular receptor α-dystroglycan to infect cells. Machupo, Guanarito, Junin and Sabia viruses are New World haemorrhagic fever viruses that do not use α-dystroglycan. Here we show a specific, high-affinity association between transferrin receptor 1 (TfR1) and the entry glycoprotein (GP) of Machupo virus. Expression of human TfR1, but not human transferrin receptor 2, in hamster cell lines markedly enhanced the infection of viruses pseudotyped with the GP of Machupo, Guanarito and Junin viruses, but not with those of Lassa or lymphocytic choriomeningitis viruses. An anti-TfR1 antibody efficiently inhibited the replication of Machupo, Guanarito, Junin and Sabia viruses, but not that of Lassa virus. Iron depletion of culture medium enhanced, and iron supplementation decreased, the efficiency of infection by Junin and Machupo but not Lassa pseudoviruses. These data indicate that TfR1 is a cellular receptor for New World haemorrhagic fever arenaviruses.
UR - http://www.scopus.com/inward/record.url?scp=33847392178&partnerID=8YFLogxK
U2 - 10.1038/nature05539
DO - 10.1038/nature05539
M3 - Article
C2 - 17287727
AN - SCOPUS:33847392178
SN - 0028-0836
VL - 446
SP - 92
EP - 96
JO - Nature
JF - Nature
IS - 7131
ER -