Transmitter regulation of voltage-dependant K+ channels expressed in Xenopus oocytes

M. P. Kavanaugh; M. J. Christie; P. B. Osborne; A. E. Busch; K. Z. Shen; Y. N.P.H. Wu Seeburg; J. P. Adelman; R. A. North

doi:10.1042/bj2770899

Transmitter regulation of voltage-dependant K⁺ channels expressed in Xenopus oocytes

M. P. Kavanaugh, M. J. Christie, P. B. Osborne, A. E. Busch, K. Z. Shen, Y. N.P.H. Wu Seeburg, J. P. Adelman, R. A. North

Oregon Health and Science University

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Voltage-dependent K⁺ channels (RBK1, RBK2 and RGK5) were co-expressed in Xenopus oocytes with 5-hydroxytryptamine (5-HT₂) receptors. K⁺ currents measured 2-4 days later were inhibited by 5-HT (100 nM-10 μM, 20-30 s application) by up to 90 %. The effect of 5-HT was mimicked by intracellular injection of Ins(1,4,5)P₃. Increasing the Ca²⁺ concentration at the inner surface of excised membrane patches did not decrease the K⁺ current.

Original language	English
Pages (from-to)	899-902
Number of pages	4
Journal	Biochemical Journal
Volume	277
Issue number	3
DOIs	https://doi.org/10.1042/bj2770899
State	Published - 1991

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title = "Transmitter regulation of voltage-dependant K+ channels expressed in Xenopus oocytes",

abstract = "Voltage-dependent K+ channels (RBK1, RBK2 and RGK5) were co-expressed in Xenopus oocytes with 5-hydroxytryptamine (5-HT2) receptors. K+ currents measured 2-4 days later were inhibited by 5-HT (100 nM-10 μM, 20-30 s application) by up to 90 %. The effect of 5-HT was mimicked by intracellular injection of Ins(1,4,5)P3. Increasing the Ca2+ concentration at the inner surface of excised membrane patches did not decrease the K+ current.",

author = "Kavanaugh, {M. P.} and Christie, {M. J.} and Osborne, {P. B.} and Busch, {A. E.} and Shen, {K. Z.} and {Wu Seeburg}, {Y. N.P.H.} and Adelman, {J. P.} and North, {R. A.}",

year = "1991",

doi = "10.1042/bj2770899",

language = "English",

volume = "277",

pages = "899--902",

journal = "Biochemical Journal",

issn = "0264-6021",

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T1 - Transmitter regulation of voltage-dependant K+ channels expressed in Xenopus oocytes

AU - Kavanaugh, M. P.

AU - Christie, M. J.

AU - Osborne, P. B.

AU - Busch, A. E.

AU - Shen, K. Z.

AU - Wu Seeburg, Y. N.P.H.

AU - Adelman, J. P.

AU - North, R. A.

PY - 1991

Y1 - 1991

N2 - Voltage-dependent K+ channels (RBK1, RBK2 and RGK5) were co-expressed in Xenopus oocytes with 5-hydroxytryptamine (5-HT2) receptors. K+ currents measured 2-4 days later were inhibited by 5-HT (100 nM-10 μM, 20-30 s application) by up to 90 %. The effect of 5-HT was mimicked by intracellular injection of Ins(1,4,5)P3. Increasing the Ca2+ concentration at the inner surface of excised membrane patches did not decrease the K+ current.

AB - Voltage-dependent K+ channels (RBK1, RBK2 and RGK5) were co-expressed in Xenopus oocytes with 5-hydroxytryptamine (5-HT2) receptors. K+ currents measured 2-4 days later were inhibited by 5-HT (100 nM-10 μM, 20-30 s application) by up to 90 %. The effect of 5-HT was mimicked by intracellular injection of Ins(1,4,5)P3. Increasing the Ca2+ concentration at the inner surface of excised membrane patches did not decrease the K+ current.

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U2 - 10.1042/bj2770899

DO - 10.1042/bj2770899

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AN - SCOPUS:0025777295

SN - 0264-6021

VL - 277

SP - 899

EP - 902

JO - Biochemical Journal

JF - Biochemical Journal

IS - 3

ER -

Transmitter regulation of voltage-dependant K⁺ channels expressed in Xenopus oocytes

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