Use of rpsL as a counterselectable marker in borrelia burgdorferi

Dan Drecktrah, J. Miles Douglas, D. Scott Samuels

Research output: Contribution to journalArticlepeer-review

Abstract

We have demonstrated that rpsL, encoding the S12 protein of the small ribosomal subunit, can be used as a counterselectable marker in Borrelia burgdorferi, the causative agent of Lyme disease. Mutations in rpsL confer streptomycin resistance. Streptomycin susceptibility is dominant in an rpsL merodiploid, and streptomycin selects for the loss of wild-type rpsL carried in trans. This is the first description of a counterselectable marker in B. burgdorferi.

Original languageEnglish
Pages (from-to)985-987
Number of pages3
JournalApplied and Environmental Microbiology
Volume76
Issue number3
DOIs
StatePublished - Feb 2010

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