@inbook{072e39d709fd493782a615cf3f81462d,
title = "Using RNAseq to Uncover Transcriptional and Splicing Differences in Host Cells During Rift Valley Fever Virus Infection",
abstract = "RNAseq is a valuable tool that can aid researchers in uncovering the transcriptional changes that occur when a viral pathogen infects a host cell. Viral infection will invariably cause differential expression of many genes, from transcription of mRNA to alternative splicing and degradation. This change in gene expression can be a result of immune activation or a direct activity of the virus to alter the host cell{\textquoteright}s environment to make it more favorable for viral replication. Studying the innate immune response to a pathogen can reveal which cellular pathways are active, indicating the steps that the host takes to halt viral infection, and detecting virus-mediated mRNA expression changes can help with identifying the pathways which may be exploited by the virus. Gene expression changes—both cell-caused and virus-caused—can be studied through RNAseq, helping to provide a clearer picture of the cellular changes that occur during viral infection. In this protocol, we outline methods to carry out mRNA sequencing in Rift Valley fever virus-infected cell cultures, from infection to library prep and analysis.",
keywords = "Alternative splicing, Gene expression, Host pathogen interaction, MP-12, Rift Valley fever virus, RNAseq, Transcriptomics",
author = "White, {Luke Adam} and Havranek, {Katherine E.} and Lodmell, {J. Stephen}",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.",
year = "2024",
doi = "10.1007/978-1-0716-3926-9_23",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "373--383",
booktitle = "Methods in Molecular Biology",
}