Using Time-Resolved Fluorescence Anisotropy of di-4-ANEPPDHQ and F2N12S to Analyze Lipid Packing Dynamics in Model Systems

Harmen B.B. Steele, Matthew J. Sydor, Donald S. Anderson, Andrij Holian, J. B.Alexander Ross

Research output: Contribution to journalArticlepeer-review

Abstract

The fluorescence probes di-4-ANEPPDHQ and F2N12S have solvochromatic emission spectra and fluorescence lifetimes that are sensitive to order within the environment of lipid membranes. We show in this communication that the time-resolved fluorescence anisotropy of these probes, analyzed either by the wobble-in-a-cone model or by the model-independent order parameter S 2 , provides complementary information about dynamics and lipid packing in a variety of homogeneous lipid membranes systems.

Original languageEnglish
JournalJournal of Fluorescence
DOIs
StateAccepted/In press - 2019

Keywords

  • F2N12S
  • Fluorescence anisotropy
  • Lipid dynamics
  • Lipid packing
  • Order parameter
  • Wobble-in-a-cone
  • di-4-ANEPPDHQ

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