Using Time-Resolved Fluorescence Anisotropy of di-4-ANEPPDHQ and F2N12S to Analyze Lipid Packing Dynamics in Model Systems

Harmen B.B. Steele; Matthew J. Sydor; Donald S. Anderson; Andrij Holian; J. B.Alexander Ross

doi:10.1007/s10895-019-02363-7

Using Time-Resolved Fluorescence Anisotropy of di-4-ANEPPDHQ and F2N12S to Analyze Lipid Packing Dynamics in Model Systems

Harmen B.B. Steele, Matthew J. Sydor, Donald S. Anderson, Andrij Holian, J. B.Alexander Ross

University of Montana

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The fluorescence probes di-4-ANEPPDHQ and F2N12S have solvochromatic emission spectra and fluorescence lifetimes that are sensitive to order within the environment of lipid membranes. We show in this communication that the time-resolved fluorescence anisotropy of these probes, analyzed either by the wobble-in-a-cone model or by the model-independent order parameter S², provides complementary information about dynamics and lipid packing in a variety of homogeneous lipid membranes systems.

Original language	English
Pages (from-to)	347-352
Number of pages	6
Journal	Journal of Fluorescence
Volume	29
Issue number	2
DOIs	https://doi.org/10.1007/s10895-019-02363-7
State	Published - Mar 2019

Keywords

Di-4-ANEPPDHQ
F2N12S
Fluorescence anisotropy
Lipid dynamics
Lipid packing
Order parameter
Wobble-in-a-cone

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10.1007/s10895-019-02363-7

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title = "Using Time-Resolved Fluorescence Anisotropy of di-4-ANEPPDHQ and F2N12S to Analyze Lipid Packing Dynamics in Model Systems",

abstract = "The fluorescence probes di-4-ANEPPDHQ and F2N12S have solvochromatic emission spectra and fluorescence lifetimes that are sensitive to order within the environment of lipid membranes. We show in this communication that the time-resolved fluorescence anisotropy of these probes, analyzed either by the wobble-in-a-cone model or by the model-independent order parameter S2, provides complementary information about dynamics and lipid packing in a variety of homogeneous lipid membranes systems.",

keywords = "Di-4-ANEPPDHQ, F2N12S, Fluorescence anisotropy, Lipid dynamics, Lipid packing, Order parameter, Wobble-in-a-cone",

author = "Steele, \{Harmen B.B.\} and Sydor, \{Matthew J.\} and Anderson, \{Donald S.\} and Andrij Holian and Ross, \{J. B.Alexander\}",

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AU - Steele, Harmen B.B.

AU - Sydor, Matthew J.

AU - Anderson, Donald S.

AU - Holian, Andrij

AU - Ross, J. B.Alexander

N1 - Publisher Copyright: © Springer Science+Business Media, LLC, part of Springer Nature 2019.

PY - 2019/3

Y1 - 2019/3

N2 - The fluorescence probes di-4-ANEPPDHQ and F2N12S have solvochromatic emission spectra and fluorescence lifetimes that are sensitive to order within the environment of lipid membranes. We show in this communication that the time-resolved fluorescence anisotropy of these probes, analyzed either by the wobble-in-a-cone model or by the model-independent order parameter S2, provides complementary information about dynamics and lipid packing in a variety of homogeneous lipid membranes systems.

AB - The fluorescence probes di-4-ANEPPDHQ and F2N12S have solvochromatic emission spectra and fluorescence lifetimes that are sensitive to order within the environment of lipid membranes. We show in this communication that the time-resolved fluorescence anisotropy of these probes, analyzed either by the wobble-in-a-cone model or by the model-independent order parameter S2, provides complementary information about dynamics and lipid packing in a variety of homogeneous lipid membranes systems.

KW - Di-4-ANEPPDHQ

KW - F2N12S

KW - Fluorescence anisotropy

KW - Lipid dynamics

KW - Lipid packing

KW - Order parameter

KW - Wobble-in-a-cone

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VL - 29

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JF - Journal of Fluorescence

IS - 2

ER -

Using Time-Resolved Fluorescence Anisotropy of di-4-ANEPPDHQ and F2N12S to Analyze Lipid Packing Dynamics in Model Systems

Abstract

Keywords

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