Using Time-Resolved Fluorescence Anisotropy of di-4-ANEPPDHQ and F2N12S to Analyze Lipid Packing Dynamics in Model Systems

  • Harmen B.B. Steele
  • , Matthew J. Sydor
  • , Donald S. Anderson
  • , Andrij Holian
  • , J. B.Alexander Ross

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The fluorescence probes di-4-ANEPPDHQ and F2N12S have solvochromatic emission spectra and fluorescence lifetimes that are sensitive to order within the environment of lipid membranes. We show in this communication that the time-resolved fluorescence anisotropy of these probes, analyzed either by the wobble-in-a-cone model or by the model-independent order parameter S2, provides complementary information about dynamics and lipid packing in a variety of homogeneous lipid membranes systems.

Original languageEnglish
Pages (from-to)347-352
Number of pages6
JournalJournal of Fluorescence
Volume29
Issue number2
DOIs
StatePublished - Mar 2019

Funding

Funder number
P20GM103546

    Keywords

    • Di-4-ANEPPDHQ
    • F2N12S
    • Fluorescence anisotropy
    • Lipid dynamics
    • Lipid packing
    • Order parameter
    • Wobble-in-a-cone

    Fingerprint

    Dive into the research topics of 'Using Time-Resolved Fluorescence Anisotropy of di-4-ANEPPDHQ and F2N12S to Analyze Lipid Packing Dynamics in Model Systems'. Together they form a unique fingerprint.

    Cite this