TY - CHAP
T1 - Voltage Clamp and Fluorometric Techniques for Studying Glutamate Transporter Function
AU - Tzingounis, Anastassios V.
AU - Larsson, H. Peter
AU - Kavanaugh, Michael P.
N1 - Publisher Copyright:
© 2002 by Wiley-Liss, Inc., Hoboken, New Jersey.
PY - 2003/1/1
Y1 - 2003/1/1
N2 - Glutamate transporters can influence the time course of synaptically released glutamate and maintain low ambient extracellular glutamate levels by accumulating it in the cell against a large gradient. Understanding these physiological processes requires a knowledge of the transporters’ kinetics and structure-function relationships. In this chapter, we outline three methods - voltage clamp fluorometry, concentration jumps to outside-out patches, and computer modeling. These techniques provide a potentially powerful approach to study kinetics and the protein conformational changes during the transport cycle.
AB - Glutamate transporters can influence the time course of synaptically released glutamate and maintain low ambient extracellular glutamate levels by accumulating it in the cell against a large gradient. Understanding these physiological processes requires a knowledge of the transporters’ kinetics and structure-function relationships. In this chapter, we outline three methods - voltage clamp fluorometry, concentration jumps to outside-out patches, and computer modeling. These techniques provide a potentially powerful approach to study kinetics and the protein conformational changes during the transport cycle.
UR - http://www.scopus.com/inward/record.url?scp=1642301368&partnerID=8YFLogxK
U2 - 10.1002/0471434043.ch13
DO - 10.1002/0471434043.ch13
M3 - Chapter
AN - SCOPUS:1642301368
SN - 9780471065135
SP - 203
EP - 215
BT - Transmembrane Transporters
PB - wiley
ER -